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Article
Expression of chitinase and chitin binding proteins (CBP’s) by Listeria monocytogenes J0161 in biofilm and Co-culture broths
African Journal of Microbiology Research (2011)
  • Prem Saran Tirumalai
  • Soam Prakash
Abstract
Listeria monocytogenes is a dreaded pathogen colonizes to form biofilm in the natural environments
and in food processing facility. Biofilm state makes the bacterium resistant to antimicrobial agents. L.
monocytogenes, an intracellular parasite also colonizes the mammalian epithelial cells in the gut.
Chitinase and other chitinolytic proteins expressed by L. monocytogenes contribute to the colonization
of the bacterium in the intestine. It has been reported that chitinase and chitin binding proteins are
essential for attachment of the bacterium to the epithelial cells. In this study, we have pursued to
analyze, if chitinase and chitin binding proteins play a role in biofilm formation and the expression of
these proteins otherwise in a co-culture state. We report the expression pattern of these proteins, which
supports our claim that chitinase and chitin binding proteins have a role to play in biofilm formation.
Keywords
  • Listeria monocytogenes,
  • chitinase,
  • microarray,
  • biofilm,
  • co-culture,
  • gene expression.
Disciplines
Publication Date
Fall October 17, 2011
Citation Information
Prem Saran Tirumalai and Soam Prakash. "Expression of chitinase and chitin binding proteins (CBP’s) by Listeria monocytogenes J0161 in biofilm and Co-culture broths" African Journal of Microbiology Research (2011)
Available at: http://works.bepress.com/soamprakash/22/