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Alteration in testicular cell components following transiently induced ischaemia in prepubertal bulls
Reproduction, Fertility and Development (2008)
  • G.M. Schuenemann
  • S.M. Lilitha Chamindrani Mendis-Handagama, University of Tennessee - Knoxville
  • T.M. Prado
  • F.N. Schrick
Abstract
The aim of the present study was to evaluate transient testicular ischaemia (induced using elastrator bands) in Jersey calves on testicular morphology and development. Treatments (at 27 ± 5 days of age) consisted of control (0 h banding) and banding for 2, 4 or 8 h (n = 4 in each group). After castration (at 60 ± 5 days of age), the right testis was used for calculation of cell components per testis according to the point-counting method. Bodyweight (59.8 ± 6.2 kg) and scrotal circumference (SC) at banding (9.1 ± 0.2 cm) did not differ between groups. Fresh testis weight, scrotal temperature immediately before band removal and daily SC growth were decreased in ischaemic (4 and 8 h) testes compared with controls (P < 0.05). In addition, the number of Sertoli and Leydig cells was significantly reduced in the 8 h ischaemic treatment group (P < 0.05). Transiently induced ischaemia significantly decreased the number of germ cells in the 8 h ischaemic treatment group (13 ± 5 × 106 cells) compared with the 0, 2 and 4 h ischaemic treatment groups (38 ± 6, 32 ± 6 and 33 ± 5 × 106 cells, respectively; P < 0.05). These results suggest that transiently induced ischaemia for 8 h significantly decreases the number of germ, Sertoli and Leydig cells in prepubertal testis.
Keywords
  • germ cell,
  • recipient animals,
  • spermatogonial transplantation,
  • testis development
Publication Date
2008
Citation Information
G.M. Schuenemann, S.M. Lilitha Chamindrani Mendis-Handagama, T.M. Prado and F.N. Schrick. "Alteration in testicular cell components following transiently induced ischaemia in prepubertal bulls" Reproduction, Fertility and Development Vol. 20 Iss. 7 (2008)
Available at: http://works.bepress.com/sm_mendis-handagama/4/