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Online ozonolysis methods for the determination of double bond position in unsaturated lipids
Faculty of Science - Papers (Archive)
  • Michael Thomas, University of Wollongong
  • Todd W Mitchell, University of Wollongong
  • Stephen J. Blanksby, University of Wollongong
RIS ID
30590
Publication Date
1-1-2009
Abstract

Modern lipidomics relies heavily on mass spectrometry for the structural characterization and quantification of lipids of biological origins. Structural information is gained by tandem mass spectrometry (MS/MS) whereby lipid ions are fragmented to elucidate lipid class, fatty acid chain length, and degree of unsaturation. Unfortunately, however, in most cases double bond position cannot be assigned based on MS/MS data alone and thus significant structural diversity is hidden from such analyses. For this reason, we have developed two online methods for determining double bond position within unsaturated lipids; ozone electrospray ionization mass spectrometry (OzESI-MS) and ozone-induced dissociation (OzID). Both techniques utilize ozone to cleave C-C double bonds that result in chemically induced fragment ions that locate the position(s) of unsaturation.

Citation Information
Michael Thomas, Todd W Mitchell and Stephen J. Blanksby. "Online ozonolysis methods for the determination of double bond position in unsaturated lipids" (2009) p. 413 - 441
Available at: http://works.bepress.com/sjblanksby/11/