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Article
Estrogen receptor α mediates acute potassium channel stimulation in human coronary artery smooth muscle cells
Journal of Pharmacology and Experimental Therapeutics
  • Guichin Han
  • Xiuping Yu
  • Luo Lu
  • Shuyi Li
  • Handong Ma
  • Shu Zhu, Philadelphia Colleg of Osteopathic Medicine
  • Xiuyun Cui
  • Richard E. White, Philadelphia College of Osteopathic Medicine
Document Type
Article
Publication Date
1-1-2006
Abstract
The pleiotropic effects of estrogen are mediated via stimulation of two estrogen receptor (ER) subtypes, ERα and ERß. Although a number of studies have identified expression of one or both subtypes in estrogen target tissues, fewer studies have correlated ER expression with a functional role of these proteins in regulating cellular excitability. In the present study, we have combined cellular fluorescence, immunocytochemistry, and molecular expression techniques with single-channel patch-clamp studies to determine which ER mediates estrogen-stimulated potassium channel activity in human coronary artery smooth muscle cells (HCASMC). We had demonstrated previously that estrogen stimulates activity of the large-conductance, calcium- and voltage-activated potassium (BKCa) channel in HCASMC via a nongenomic mechanism. We now demonstrate expression of both ERα and ERß subtypes in HCASMC. Functionally, however, expression of ERα antisense plasmid abolished the acute effect of estrogen on these channels, whereas estrogen retained its ability to stimulate BKCa channels in cells transfected with only green fluorescence protein. In contrast, overexpression of ERα enhanced the stimulatory action of estrogen in HCASMC. Transfection with ERα antisense/sense plasmid did not alter ERß expression. These findings indicate that the ERα isoform mediates estrogen-induced stimulation of BKCa channels in HCASMC and thereby provide evidence for a receptor-dependent signaling mechanism that can mediate estrogen-induced inhibition of cellular excitability.
Comments

This article was published in Journal of Pharmacology and Experimental Therapeutics, Volume 316, Issue 3, Pages 1025-1030.

The published version is available at http://dx.doi.org/10.1124/jpet.105.093542.

Copyright © 2006 Scopus.

Citation Information
Guichin Han, Xiuping Yu, Luo Lu, Shuyi Li, et al.. "Estrogen receptor α mediates acute potassium channel stimulation in human coronary artery smooth muscle cells" Journal of Pharmacology and Experimental Therapeutics Vol. 316 Iss. 3 (2006) p. 1025 - 1030
Available at: http://works.bepress.com/shu_zhu/10/