Sudden death syndrome (SDS) caused by Fusarium virguliforme (FV) formerly F. solani f. sp. glycines is one of the major yield reducing diseases of soybean (Glycine max). There is concern among producers due to noticeable expansion in geographical array of SDS and the associated potential yield losses. Yield loss due to SDS in the top 10 soybean producing countries varies from 2x103 in Canada to 0x105metric tons in the United States (Wrather et al., 2001). Techniques developed earlier for SDS resistance screening were inconsistent in foliar symptoms expression. Hence, objectives of the study during 2002–2005 were to develop a simple, rapid, repeatable, quantifiable and season independent resistance screening technique to measure relative tolerance of soybean varieties to SDS and understand infection process.