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Determination of Carboplatin in Canine Plasma by High-performance Liquid Chromatography
Biomedical Chromatography (2010)
  • Nicolas Villarino, University of Tennessee - Knoxville
  • Sherry Cox, University of Tennessee - Knoxville
  • Jason Yarbrough, University of Tennessee - Knoxville
  • Tomas Martin-Jimenez, University of Tennessee, Knoxville
Abstract
Carboplatin is an antineoplastic drug administered to treat different tumoral conditions in canine oncology. The objective of this study was to validate a high-performance chromatographic (HPLC) method which could be applied in canine pharmacokinetic studies. Following ultrafiltration using a Centrifree device, standards, quality controls and plasma samples were separated by isocratic reversed-phase HPLC on an Inertsil ODS-2 (250 × 4.6 mm i.d.) analytical column and quantified using UV detection at 220 nm. The mobile phase was potassium phosphate (pH 4.5), with a flow-rate of 1.0 mL/min. The procedure produced a linear curve (r2 > 0.999) over the concentration range 1–200 μg/mL. The lower limit of quantification was 1 μg/mL. The intra-assay and inter-assay precision was ∼90%. The overall recovery was ∼90%. The method was illustrated with a preliminary pharmacokinetic analysis on nine dogs treated with carboplatin at our hospital. Carboplatin disposition followed a monocompartmental structure in dogs and was characterized by a short half-life (50 min).
Keywords
  • carboplatin; dog; validation; HPLC; pharmacokinetics
Publication Date
August, 2010
Citation Information
Nicolas Villarino, Sherry Cox, Jason Yarbrough and Tomas Martin-Jimenez. "Determination of Carboplatin in Canine Plasma by High-performance Liquid Chromatography" Biomedical Chromatography Vol. 24 Iss. 8 (2010)
Available at: http://works.bepress.com/sherry_cox/16/