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Article
Percentage of {Gamma}{Delta} T Cells in Panniculitis by Paraffin Immunohistochemical Analysis.
American journal of clinical pathology
  • Michele Roullet
  • Shereen M F Gheith, MD, PhD, Lehigh Valley Health Network
  • Joanne Mauger
  • Jacqueline M Junkins-Hopkins
  • John K Choi
Publication/Presentation Date
6-1-2009
Abstract

Cutaneous T-cell lymphomas with panniculitis-like histologic features have different clinical courses depending on whether they are composed of alphabeta T cells or gammadelta T cells, necessitating their distinction for proper prognostication. However, unlike alphabeta T cells, gammadelta T cells cannot be reliably detected in formalin-fixed, paraffin-embedded sections. We demonstrated that a commercially available antibody can detect gammadelta T cells and examined 2 cases of flow cytometry-proven gammadelta T-cell lymphomas and 15 control cases of nonneoplastic panniculitis. In both lymphomas, the atypical lymphocytes were gammadelta T cells, whereas the reactive lymphocytes were alphabeta T cells. In contrast, nonneoplastic panniculitis had predominantly alphabeta T cells with many fewer and individually scattered gammadelta T cells. The detection of gammadelta T cells in paraffin sections provides a powerful new tool to characterize T cells in lymphomas and inflammation.

Disciplines
PubMedID
19461088
Document Type
Article
Citation Information

Roullet, M., Gheith, S. F., Mauger, J., Junkins-Hopkins, J. M., & Choi, J. K. (2009). Percentage of {gamma}{delta} T cells in panniculitis by paraffin immunohistochemical analysis. American Journal Of Clinical Pathology, 131(6), 820-826. doi:10.1309/AJCPMG37MXKYPUBE