Wu, J. N., Gheith, S., Bezman, N. A., Liu, Q., Fostel, L. V., Swanson, A. M., & ... Peterson, E. J. (2006). Adhesion- and degranulation-promoting adapter protein is required for efficient thymocyte development and selection. Journal Of Immunology (Baltimore, Md.: 1950), 176(11), 6681-6689.
Adhesion- and Degranulation-Promoting Adapter Protein is Required for Efficient Thymocyte Development and Selection.Journal of immunology (Baltimore, Md. : 1950)
AbstractAdhesion- and degranulation-promoting adapter protein (ADAP) is required in TCR-induced activation and proliferation of peripheral T cells. Loss of ADAP also impairs TCR-initiated inside-out activation of the integrin LFA-1 (CD11a/CD18, alphaLbeta2). In this study, we demonstrate that ADAP-deficient CD4/CD8 double-positive (DP) cells have a diminished ability to proliferate, and that these DP thymocytes up-regulate CD69 poorly in vivo. Moreover, in both MHC class I- and class II-restricted TCR transgenic models, loss of ADAP interferes with both positive and negative selection. ADAP deficiency also impairs the ability of transgene-bearing DP thymocytes to form conjugates with Ag-loaded presenting cells. These findings suggest that ADAP is critical for thymocyte development and selection.