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Article
The human phosphotyrosine signaling network: evolution and hotspots of hijacking in cancer.
Genome Research
  • Lei Li, University of Western Ontario
  • Chabane Tibiche, Biotechnology Research Institute, National Research Council Canada
  • Cong Fu, Biotechnology Research Institute, National Research Council Canada
  • Tomonori Kaneko, University of Western Ontario
  • Michael F. Moran, University of Toronto
  • Martin Schiller, University of Nevada, Las Vegas
  • Shawn Shun-Cheng Li, University of Western Ontario
  • Edwin Wang, Biotechnology Research Institute, National Research Council Canada
Document Type
Article
Publication Date
7-1-2012
Abstract

Phosphotyrosine (pTyr) signaling, which plays a central role in cell-cell and cell-environment interactions, has been considered to be an evolutionary innovation in multicellular metazoans. However, neither the emergence nor the evolution of the human pTyr signaling system is currently understood. Tyrosine kinase (TK) circuits, each of which consists of a TK writer, a kinase substrate, and a related reader, such as Src homology (SH) 2 domains and pTyr-binding (PTB) domains, comprise the core machinery of the pTyr signaling network. In this study, we analyzed the evolutionary trajectories of 583 literature-derived and 50,000 computationally predicted human TK circuits in 19 representative eukaryotic species and assigned their evolutionary origins. We found that human TK circuits for intracellular pTyr signaling originated largely from primitive organisms, whereas the inter- or extracellular signaling circuits experienced significant expansion in the bilaterian lineage through the "back-wiring" of newly evolved kinases to primitive substrates and SH2/PTB domains. Conversely, the TK circuits that are involved in tissue-specific signaling evolved mainly in vertebrates by the back-wiring of vertebrate substrates to primitive kinases and SH2/PTB domains. Importantly, we found that cancer signaling preferentially employs the pTyr sites, which are linked to more TK circuits. Our work provides insights into the evolutionary paths of the human pTyr signaling circuits and suggests the use of a network approach for cancer intervention through the targeting of key pTyr sites and their associated signaling hubs in the network.

Keywords
  • Animals,
  • Cancer--Treatment,
  • Cell Communication,
  • Cell interaction,
  • Cell Membrane/genetics,
  • Cell Membrane/metabolism,
  • Cellular signal transduction,
  • Cytoplasm/genetics,
  • Cytoplasm/metabolism,
  • Eukaryotic cells,
  • Evolution,
  • Molecular,
  • Humans,
  • Models,
  • Genetic,
  • Molecular evolution,
  • Neoplasms/genetics,
  • Neoplasms/metabolism,
  • Organ Specificity,
  • Phosphorylation,
  • Phosphotyrosine/classification,
  • Phosphotyrosine/genetics,
  • Phosphotyrosine/metabolism,
  • Phylogeny,
  • Protein Binding,
  • Protein-Tyrosine Kinases/genetics,
  • Protein-Tyrosine Kinases/metabolism,
  • Protein-tyrosine phosphatase,
  • Signal Transduction,
  • src Homology Domains,
  • Tumors
Language
English
Citation Information
Lei Li, Chabane Tibiche, Cong Fu, Tomonori Kaneko, et al.. "The human phosphotyrosine signaling network: evolution and hotspots of hijacking in cancer." Genome Research Vol. 22 Iss. 7 (2012) p. 1222 - 1230
Available at: http://works.bepress.com/shawn-li/2/