Article
Quantitative Proteomics Identify Molecular Targets That Are Crucial in Larval Settlement and Metamorphosis of Bugula neritina
Journal of Proteome Research
(2011)
Abstract
The marine invertebrate Bugula neritina has a biphasic life cycle that consists of a swimming larval stage and a sessile juvenile and adult stage. The attachment of larvae to the substratum and their subsequent metamorphosis have crucial ecological consequences. Despite many studies on this species, little is known about the molecular mechanism of these processes. Here, we report a comparative study of swimming larvae and metamorphosing individuals at 4 and 24 h postattachment using label-free quantitative proteomics. We identified more than 1100 proteins at each stage, 61 of which were differentially expressed. Specifically, proteins involved in energy metabolism and structural molecules were generally down-regulated, whereas proteins involved in transcription and translation, the extracellular matrix, and calcification were strongly up-regulated during metamorphosis. Many tightly regulated novel proteins were also identified. Subsequent analysis of the temporal and spatial expressions of some of the proteins and an assay of their functions indicated that they may have key roles in metamorphosis of B. neritina. These findings not only provide molecular evidence with which to elucidate the substantial changes in morphology and physiology that occur during larval attachment and metamorphosis but also identify potential targets for antifouling treatment.
Keywords
- Biofouling,
- Bugula neritina,
- Label-free quantitative proteomics,
- Larval attachment and metamorphosis
Disciplines
Publication Date
2011
DOI
DOI: 10.1021/pr100817v
Publisher Statement
Published by the American Chemical Society
Citation Information
Shawn M. Arellano, Huoming Zhang, Yue Him Wong, Zhangfan Chen, et al.. "Quantitative Proteomics Identify Molecular Targets That Are Crucial in Larval Settlement and Metamorphosis of Bugula neritina" Journal of Proteome Research Vol. 10 Iss. 1 (2011) p. 349 - 360 Available at: http://works.bepress.com/shawn-arellano/10/