Objectives: This study used an ecological approach to understand the relationship between PA environment and PA behavior among rural/low-income children. Methods: Parents (N = 298) of 4th-graders completed questionnaires about their child’s PA behaviors and school environment. Descriptive, bivariate, and regression analyses were conducted. Results: One-third of students met PA recommendations (M=4.67 days, SD=2.095). Regression analyses revealed PE, participation in team sports, and race/ethnicity were all related to PA (R2 =0.22, p < .0001). Conclusion: This study revealed the importance of PE and team sports in PA levels among rural/low-income children. PA environments should be a major priority on public health policy agendas in rural communities, with an emphasis on school-based PE and access to extracurricular team sports.