Nanomaterials possess many unique properties useful for biomedical applications ranging from preoperative and intraoperative imaging to coatings, assays, and cell scaffolds. Their popularity and production value are full of competitiveness. Their related methodologies and instruments are also created or emphasized as the mainstream. Since multifunctional nanomaterials attract interests and attention of researchers, engineers, and businessmen in biomedical engineering, this journal is set up to publish a special issue devoted to this topic. The result is a collection of five original research articles, whose authors belong to academic or research institutions of five different countries from Asia, Europe, and America. Papers are representative of a large share of biomaterials with multiple functions, applications and syntheses, and characteristic methods.