Intraperitoneal Radio-localization of Tumors pre-targeted by biotinylated monoclonal AntibodiesInternational Journal of Cancer
AbstractWe describe 2-step and 3-step strategies for intraperitoneal tumor radio-localization by means of monoclonal antibodies (MAbs). Nude mice bearing intraperitoneal human colon carcinoma tumors were injected i.p. with biotinylated MAb AUAI, followed 24 hr later by radioiodinated streptavidin (2-step). The uptake of radioactivity in tumor and normal tissues was measured 4 hr after injection of radioactive compound. A 3-step strategy consisted in administering biotinylated antibody, cold avidin after 24 hr and 111In-labelled biotin after a further 4 hr; mice were then killed 2 hr later. Tumor localization of intraperitoneally-administered biotinylated antibody and direct targeting of radioactive streptavidin to biotinylated antibody bound to tumor sites were demonstrated using immunohistochemistry and autoradiography. Our results show that (i) the 2-step approach increased the percentage of radioactivity uptake by tumor with respect to directly labelled antibodies (24% vs. 6%) and improved the tumor/non-tumor ratio; (ii) the 3-step approach allowed faster blood clearance of the radioactive probe (111In-biotin) and yielded high tumor/non-tumor ratios. “Pre-targeting” methods appear to have advantages over the conventional 1-step approach with directly radiolabelled antibody.
Citation InformationG. Paganelli, Shahid Pervez, A. G. Siccardi, G. Rowlinson, et al.. "Intraperitoneal Radio-localization of Tumors pre-targeted by biotinylated monoclonal Antibodies" International Journal of Cancer Vol. 45 Iss. 6 (1990) p. 1184 - 1189
Available at: http://works.bepress.com/shahid_pervez/123/