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Article
Gastric juice for the diagnosis of H pylori infection in patients on proton pump inhibitors
World Journal of Gastroenterology
  • Javed Yakoob, Aga Khan University
  • Shahid Rasool, Aga Khan University
  • Z Abbas, Agha Khan University
  • Wasim Jafri, Aga Khan University
  • Shahab Abid, Aga Khan University
  • Muhammad Islam, Aga Khan University
  • Zubair Ahmad, Aga Khan University
Publication Date
3-14-2008
Document Type
Article
Abstract
Aim:To determine the efficacy of gastric juice polymerase chain reaction (PCR) for the detection of H pylori infection in comparison with histology and gastric antral biopsy PCR in Patients on a proton pump inhibitor (PPI). Methods: Eighty-five consecutive Patients with dyspeptic symptoms were enrolled. Gastric biopsies for histology, PCR and gastric juice were collected at endoscopy for PCR of the H pylori urease C gene (ure C). Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), accuracy, positive and negative likelihood ratio for PCR of gastric juice for the H pylori ure C gene was compared to histology and gastric antral biopsy H pylori ure C PCR in Patients with and without PPI. Results: Gastric juice PCR was positive in 66 (78%) Patients. Histology showed H pylori associated gastritis in 57 (67%). Gastric biopsy PCR was positive in 72 (85%). In Patients not taking PPI, the sensitivity, specificity, PPV, NPV, accuracy and positive and negative likelihood ratio for gastric juice PCR were 89%, 72%, 91%, 67%, 90%, 85%, 3.1 and 0.1 respectively. In Patients on PPI these values were 86%, 100%%, 100%, 29%, 86%, 9.5 and 1.4, respectively. Conclusion: Gastric juice PCR for the diagnosis of H pylori infection has increased sensitivity compared to histology with PPI. The use of gastric juice PCR is recommended to confirm H pylori status in Patients taking PPIs.
Citation Information
Javed Yakoob, Shahid Rasool, Z Abbas, Wasim Jafri, et al.. "Gastric juice for the diagnosis of H pylori infection in patients on proton pump inhibitors" World Journal of Gastroenterology Vol. 14 Iss. 10 (2008) p. 1539 - 1543
Available at: http://works.bepress.com/shahab_abid/9/