Skip to main content
Article
Comparison of biopsy-based methods for the detection of Helicobacter pylori infection
British Journal of Biomedical Science
  • Javed Yakoob, Aga Khan University
  • Muhammad Islam, Aga Khan University
  • Shahab Abid, Aga Khan University
  • Wasim Jafri, Aga Khan University
  • Zaigham Abbas, Aga Khan University
  • Z. Ahmad, Aga Khan University
Publication Date
1-1-2006
Document Type
Article
Abstract

Various biopsy-based methods for the detection of Helicobacter pylori are evaluated to determine their sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV), followed by polymerase chain reaction (PCR) for the 16S ribosomal RNA (rRNA) gene of H. pylori (16S PCR) to confirm the results. Seventy-five patients (65% [49] males, age range: 17–77 years, mean 42±14.6 years) with dyspeptic symptoms are included in the study. Gastric antrum biopsy specimens collected during endoscopy are tested using a urea agar base enriched with 40% urea solution (eUAB, Oxoid)), a commercial rapid urease test (Pronto Dry, Medical Instrument Corp, Switzerland), histopathology and 16S PCR. The eUAB test showed 97% sensitivity, 86% specificity, 84% PPV, 97% NPV and 91% accuracy when the diagnosis of H. pylori infection was made with positive Pronto Dry and histopathology. Pronto Dry showed 100% sensitivity, 82% specificity, 80% PPV, 100% NPV and 89% accuracy when the diagnosis of H. pylori infection was made on positive histopathology and eUAB. Thus, the eUAB can be used as a rapid urease test. It is economical and has a sensitivity and specificity comparable to a commercially available rapid urease test to detect urease activity of H. pylori in gastric biopsy.

Citation Information
Javed Yakoob, Muhammad Islam, Shahab Abid, Wasim Jafri, et al.. "Comparison of biopsy-based methods for the detection of Helicobacter pylori infection" British Journal of Biomedical Science Vol. 63 Iss. 4 (2006) p. 159 - 162
Available at: http://works.bepress.com/shahab_abid/5/