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Article
The e-incubator: a magnetic resonance imaging-compatible mini incubator.
Tissue Engineering Part C: Methods
  • Shadi F. Othman, University of the Pacific
  • Karin Wartella
  • Vahid Khalilzad-Sharghi, University of Nebraska–Lincoln
  • Huihui Xu, University of the Pacific
Document Type
Article
DOI
10.1089/ten.tec.2014.0273
Publication Date
4-1-2015
Abstract

The tissue engineering community has been vocal regarding the need for noninvasive instruments to assess the development of tissue-engineered constructs. Medical imaging has helped fulfill this role. However, specimens allocated to a test tube for imaging cannot be tested for a prolonged period or returned to the incubator. Therefore, samples are essentially wasted due to potential contamination and transfer in a less than optimal growth environment. In turn, we present a standalone, miniature, magnetic resonance imaging-compatible incubator, termed the e-incubator. This incubator uses a microcontroller unit to automatically sense and regulate physiological conditions for tissue culture, thus allowing for concurrent tissue culture and evaluation. The e-incubator also offers an innovative scheme to study underlying mechanisms related to the structural and functional evolution of tissues. Importantly, it offers a key step toward enabling real-time testing of engineered tissues before human transplantation. For validation purposes, we cultured tissue-engineered bone constructs for 4 weeks to test the e-incubator. Importantly, this technology allows for visualizing the evolution of temporal and spatial morphogenesis. In turn, the e-incubator can filter deficient constructs, thereby increasing the success rate of implantation of tissue-engineered constructs, especially as construct design grows in levels of complexity to match the geometry and function of patients' unique needs.

Citation Information
Shadi F. Othman, Karin Wartella, Vahid Khalilzad-Sharghi and Huihui Xu. "The e-incubator: a magnetic resonance imaging-compatible mini incubator." Tissue Engineering Part C: Methods Vol. 21 Iss. 4 (2015) p. 347 - 355 ISSN: 1937-3392
Available at: http://works.bepress.com/shadi-othman/9/