Presentation
Fingerprint and stability of terpenes in by-product wax from commercial cannabidiol production
ARD Research Symposium 2022
(2022)
Abstract
The contemporary increasing recreational and therapeutic use of cannabinoids has greatly increased hemp cultivation to satisfy demands from the rapidly growing cannabidiol (CBD) market. Cannabis sativa plant has not only cannabinoids as crucial compounds but also terpenes contribute to the pharmacology, efficacy, aroma, and flavor. As an important component, analyzing the terpenoid composition and stability in hemp wax left after CBD production would have paramount implication future applications. Thus, this study aimed to investigate terpenes composition and stability in a by-product wax from commercial cannabidiol production for further organic fruit coating preparation. A total of 104 wax samples were collected, and all extractions were performed by accelerated solvent extraction apparatus using an ASE 350 with 20ml stain steel cells. The chemical compositions of the wax extracts were characterized and quantitated by employing gas chromatography mass spectrometry (GC−MS). A total of 125 terpenes of which several monoterpenes (24.1%), diterpenes (6.56%) and sesquiterpenes (69.34%) were identified. Terpenes such as caryophyllene, guaiol, selina-3,7(11)-dien, α-Eudesmol and α-Bisabolol were commonly found among the extracts. There was a significant difference in thermal stability between monoterpenes and sesquiterpenes. Most of the monoterpenes and the sesquiterpenes showed significant decrease by 60% and 37%, respectively before their flashpoint. Even under low temperature the α-pinene and β-pinene were less stable with their relative contents of lower than 10%. Results of this study will be helpful in the next evaluation of these compound in cancer research and use as a natural insect repellent, pest control and preparation of organic fruit coatings. Furthermore, compositional information of these by-products may provide manufacturers with the opportunity to optimize processing conditions.
Disciplines
Publication Date
Spring April 2, 2022
Citation Information
Addisie Geremew, Elisha Peace, Laura Carson, Selamawit Woldesenbet, et al.. "Fingerprint and stability of terpenes in by-product wax from commercial cannabidiol production" ARD Research Symposium 2022 (2022)
Available at: http://works.bepress.com/addisie-geremew/14/