Presentation
Dissecting hemp (Cannabis sativa L.) strains variation in terpenes profile: a metabolomic approach
https://wwwcp.umes.edu/ard/1890symposium/
(2022)
Abstract
Interest in hemp (Cannabis sativa L.) as a multi-purpose crop is growing in the US. However, prolonged domestication and intensive breeding, complicating the identification among hemp strains. To meet these challenges and support the future viability and sustainability of a hemp industry understanding the chemical fingerprints of hemp strains is important. In the present study, metabolomic approach was used to characterize and screen hemp genotypes through dissecting variation in terpenoids composition. In doing so, inflorescence of 32 hemp strains were collected and dried samples were extracted with methanol by sonication followed by maceration. Terpene’s profile of each strain was characterized and quantified using gas chromatography mass spectrometry (GC−MS). We profiled 136 terpenoids accounting 24 monoterpenoids, 108 sesquiterpenoids, and 4 diterpenoids. Alpha-bergamotene, phytol, α-bisabolol, caryophyllene, caryophyllene oxide, α-bisabolene, limonene and α-pinene were the abundant terpenes across hemp strains. 96% of variation in terpenes composition detected among strains. The remaining 4% variation was between individuals of a strain highlighting potential seed source difference. Hemp strains such as blue genius open pollinated (43%), Lindora (35%), Blue Kross (35%) and Mix John (32%) were found to have large number of terpenes. This comprehensive profile of bioactive terpenes can form a baseline of reference values useful for hemp breeding, product development and clinical studies to understand the “entourage effect” of cannabis.
Disciplines
Publication Date
Spring April 2, 2022
Citation Information
Addisie Geremew, Laura Carson, Selamawit Woldesenbet and Aruna Weerasooriya. "Dissecting hemp (Cannabis sativa L.) strains variation in terpenes profile: a metabolomic approach" ARD Research Symposium 2022 (2022)
Available at: http://works.bepress.com/addisie-geremew/13/