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Article
Differential DNA Amplification and Copy Number Control in the Hypotrichous Ciliate Euplotes crassus
Journal of Protozoology
  • Scott Everet Baird, Wright State University - Main Campus
  • Lawrence A. Klobutcher
Document Type
Article
Publication Date
3-1-1991
Abstract
During macronuclear development in hypotrichous ciliated protozoans, several thousand macronuclear DNA molecules are amplified several-hundred fold. We investigated the regulation of this amplification by determining the copy numbers of three different macronuclear DNA molecules in the hypotrichous ciliate Euplotes crassus. Two of the macronuclear DNA molecules were present in approximately 1,000 copies per cell, while the third was present in approximately 6,500 copies per cell. These reiteration levels were achieved either during macronuclear development, or shortly thereafter, and were maintained during vegetative growth. The most abundant macronuclear DNA molecule is present as a single-copy sequence in the micronuclear genome. Thus, its high copy number results from differential amplification. These results indicate that DNA amplification during macronuclear development is regulated individually for each macronuclear DNA molecule.
DOI
10.1111/j.1550-7408.1991.tb06033.x
Citation Information
Scott Everet Baird and Lawrence A. Klobutcher. "Differential DNA Amplification and Copy Number Control in the Hypotrichous Ciliate Euplotes crassus" Journal of Protozoology Vol. 38 Iss. 2 (1991) p. 136 - 140 ISSN: 10665234
Available at: http://works.bepress.com/scott_baird/12/