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Article
Crystallization and preliminary X-ray analysis of the Plasmodium falciparum apicoplast DNA polymerase
Acta Crystallographica Section F:Structural Biology Communications
  • Morgan Eilise Milton, Iowa State University
  • Jun-yong Choe, Rosalind Franklin University of Medicine and Science
  • Richard B. Honzatko, Iowa State University
  • Scott W. Nelson, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
3-1-2015
DOI
10.1107/S2053230X15002423
Abstract

Infection by the parasite Plasmodium falciparum is the leading cause of malaria in humans. The parasite has a unique and essential plastid-like organelle called the apicoplast. The apicoplast contains a genome that undergoes replication and repair through the action of a replicative polymerase (apPOL). apPOL has no direct orthologs in mammalian polymerases and is therefore an attractive antimalarial drug target. No structural information exists for apPOL, and the Klenow fragment of Escherichia coli DNA polymerase I, which is its closest structural homolog, shares only 28% sequence identity. Here, conditions for the crystallization of and preliminary X-ray diffraction data from crystals of P. falciparum apPOL are reported. Data complete to 3.5 Å resolution were collected from a single crystal (2 × 2 × 5 µm) using a 5 µm beam. The space group P6522 (unit-cell parameters a = b = 141.8, c = 149.7 Å, α = β = 90, γ = 120°) was confirmed by molecular replacement. Refinement is in progress.

Comments

This article is from Acta Crystallographica Section F:Structural Biology Communications 71 (2015): 333, doi: 10.1107/S2053230X15002423. Posted with permission.

Copyright Owner
International Union of Crystallography
Language
en
File Format
application/pdf
Citation Information
Morgan Eilise Milton, Jun-yong Choe, Richard B. Honzatko and Scott W. Nelson. "Crystallization and preliminary X-ray analysis of the Plasmodium falciparum apicoplast DNA polymerase" Acta Crystallographica Section F:Structural Biology Communications Vol. F71 Iss. 3 (2015) p. 333 - 337
Available at: http://works.bepress.com/scott-nelson/6/