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Article
Soy isoflavones have an antiestrogenic effect and alter mammary promoter hypermethylation in healthy premenopausal women1
Nutrition and Cancer
  • Wenyi Qin, University of Missouri School of Medicine
  • Weizhu Zhu, University of Missouri School of Medicine
  • Huidong Shi, University of Missouri School of Medicine
  • John E. Hewett, University of Missouri School of Medicine
  • Rachel L. Ruhlen, University of Missouri School of Medicine
  • Ruth S. MacDonald, Iowa State University
  • George E. Rottinghaus, University of Missouri School of Medicine
  • Yin-Chieh Chien, University of Missouri School of Medicine
  • Edward R. Sauter, University of Missouri School of Medicine
Document Type
Article
Publication Date
2-21-2009
DOI
10.1080/01635580802404196
Abstract

We hypothesized that soy isoflavones would have dose related estrogenic and methylation effects. 34 healthy premenopausal women were prospectively enrolled and randomized in double-blind fashion to receive either 40 mg or 140 mg isoflavones daily through one menstrual cycle. Breast specific (NAF) and systemic (serum) estrogenic effects were assessed measuring the estrogenic marker complement (C)3 and changes in cytology, while methylation effects were evaluated in mammary ductoscopy (MD) specimens using methylation specific PCR assessment of five genes (p16, RASSF1A, RARβ2, ER, and CCND2) associated with breast carcinogenesis. Serum genistein significantly increased post treatment in women consuming both isoflavone doses. Neither NAF nor MD cytology significantly changed after either low or high dose isoflavones. Serum C3 levels post treatment were inversely related to change in serum genistein (r= -0.76, p=0.0045) in women consuming low dose isoflavones. RARβ2 hypermethylation increased post treatment correlated with the post treatment level of genistein among all subjects (r=0.67, p=0.0017) and in women receiving high dose isoflavones (r=0.68, p=0.021). At the low dose, CCND2 hypermethylation increase correlated with post treatment genistein levels (r=0.79, p=0.011). The inverse correlation between C3 and genistein suggests an antiestrogenic effect. Isoflavones induced dose specific changes in RARβ2 and CCND2 gene methylation which correlated with genistein levels. This work provides novel insights into estrogenic and methylation effects of dietary isoflavones. 2

Comments

This is a manuscript of an article from Nutrition and Cancer. 61(2): 2009, 238-244. doi: 10.1080/01635580802404196. Posted with permission.

Copyright Owner
Taylor & Francis
Language
en
Date Available
2014/10/22
File Format
application/pdf
Citation Information
Wenyi Qin, Weizhu Zhu, Huidong Shi, John E. Hewett, et al.. "Soy isoflavones have an antiestrogenic effect and alter mammary promoter hypermethylation in healthy premenopausal women1" Nutrition and Cancer Vol. 61 Iss. 2 (2009) p. 238 - 244
Available at: http://works.bepress.com/ruth_macdonald/6/