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Article
Site-directed mutagenesis and PBAN activation of the Helicoverpa zea PBAN-receptor
FEBS Letters
  • Man-Yeon Choi, United States Department of Agriculture
  • Russell A. Jurenka, Iowa State University
Document Type
Article
Disciplines
Publication Date
3-1-2010
DOI
10.1016/j.febslet.2010.02.033
Abstract

Pheromone biosynthesis-activating neuropeptide (PBAN) and pyrokinins belong to a family of insect peptide hormones that have a common FXPRLamide C-terminal ending. The G-protein-coupled receptors (GPCRs) for this peptide family were first identified from a moth and Drosophila with sequence similarity to neuromedin U receptors from vertebrates. We have characterized the PBAN-receptor (PBAN-R or PR) active binding domains using chimeric GPCRs and proposed that extracellular loop 3 is critical for ligand selection. Here, we characterized the 3rd extracellular domain of PBAN-R through site-directed point mutations. Results are discussed in context of the structural features required for receptor activation using receptor activation experiments and in silico computational modeling. This research will help in characterizing these receptors towards a goal of finding agonists and/or antagonists for PBAN/pyrokinin receptors.

Comments

This article is from FEBS Letters 584 (2010): 1212, doi:10.1016/j.febslet.2010.02.033. Posted with permission.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
Date Available
2014-10-16
File Format
application/pdf
Citation Information
Man-Yeon Choi and Russell A. Jurenka. "Site-directed mutagenesis and PBAN activation of the Helicoverpa zea PBAN-receptor" FEBS Letters Vol. 584 (2010) p. 1212 - 1216
Available at: http://works.bepress.com/russell_jurenka/9/