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Article
Identification and characterization of the pyrokinin/pheromone biosynthesis activating neuropeptide family of G protein-coupled receptors from Ostrinia nubilalis
Insect Molecular Biology
  • Tyasning Nusawardani, Iowa State University
  • J. A. Kroemer, Iowa State University
  • Man-Yeon Choi, United States Department of Agriculture
  • Russell A. Jurenka, Iowa State University
Document Type
Article
Disciplines
Publication Date
6-1-2013
DOI
10.1111/imb.12025
Abstract

Insects have two closely related G protein-coupled receptors belonging to the pyrokinin/pheromone biosynthesis activating neuropeptide (pyrokinin/PBAN) family, one with the ligand PBAN or pyrokinin-2 and another with diapause hormone or pyrokinin-1 as a ligand. A related receptor is activated by products of the capa gene, periviscerokinins. Here we characterized the PBAN receptor and the diapause hormone receptor from the European corn borer, Ostrinia nubilalis. We also identified a partial sequence for the periviscerokinin receptor. Quantitative PCR of mRNA for all three receptors indicated differential expression in various life stages and tissues. All three splice variants of the PBAN receptor were identified with all variants found in pheromone gland tissue. Immunohistochemistry of V5 tags of expressed receptors indicated that all three variants and the diapause hormone receptor were expressed at similar levels in Spodoptera frugiperda 9 (Sf9) cells. However, the A- and B-variants were not active in our functional assay, which confirms studies from other moths. Functional expression of the C-variant indicated that it is has a 44 nM half effective concentration for activation by PBAN. The diapause hormone receptor was activated by diapause hormone with a 150 nM half effective concentration.

Comments

This article is from Insect Molecular Biology 22 (2013): 331, doi:10.1111/imb.12025. Posted with permission.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
Date Available
2014-10-16
File Format
application/pdf
Citation Information
Tyasning Nusawardani, J. A. Kroemer, Man-Yeon Choi and Russell A. Jurenka. "Identification and characterization of the pyrokinin/pheromone biosynthesis activating neuropeptide family of G protein-coupled receptors from Ostrinia nubilalis" Insect Molecular Biology Vol. 22 Iss. 3 (2013) p. 331 - 340
Available at: http://works.bepress.com/russell_jurenka/7/