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Article
Regulation of pheromone biosynthesis by a brain hormone in two moth species
Proceedings of the National Academy of Sciences USA (1989)
  • Juliet D. Tang, Cornell University
  • Ralph E. Charlton, Cornell University
  • Russell A. Jurenka, Iowa State University
  • Walter A. Wolf, Cornell University
  • P. Larry Phelan, Ohio State University
  • Leam Sreng, Cornell University
  • Wendell L. Roelofs, Cornell University
Abstract
Experiments were performed to characterize the action of a brain hormone on pheromone biosynthesis in female redbanded leafroller and cabbage looper moths. Results showed that the two species differed in their respective control mechanisms. In the cabbage looper, pheromone titer from decapitated females that received either saline or brain extract injections was not significantly different from control females, suggesting that pheromone biosynthesis was not dependent on the presence of the brain hormone. In contrast, with redbanded leafroller females, studies using radiolabeled acetate incorporation as well as incorporation of deuterium-labeled hexadecanoic acid showed that (i) the brain hormone was required for pheromone biosynthesis, (ii) the brain hormone regulated pheromone biosynthesis by activating synthesis of octadecanoyl and hexadecanoyl intermediates, and (iii) the brain hormone did not control other enzymes in the pathway. Regulation of fatty acid synthetase was unlikely since assays of the enzyme from decapitated and normal females showed no differences in the amount or distribution of the 18- and 16-carbon acyl end products. These results in conjunction with those from organ cultures of the pheromone gland suggest that the brain hormone acts by increasing the substrate supply for fatty acid synthesis.
Keywords
  • neuroendocrine regulation,
  • pheromone biosynthesis,
  • fatty acid synthesis,
  • Argryrotaenia velutinana,
  • Trichoplusia ni
Disciplines
Publication Date
March, 1989
Publisher Statement
This article is from Proceedings of the National Academy of Sciences USA86(1989): 1806-1810, doi:10.1073/pnas.86.6.1806.
Citation Information
Juliet D. Tang, Ralph E. Charlton, Russell A. Jurenka, Walter A. Wolf, et al.. "Regulation of pheromone biosynthesis by a brain hormone in two moth species" Proceedings of the National Academy of Sciences USA Vol. 86 Iss. 6 (1989)
Available at: http://works.bepress.com/russell_jurenka/3/