A field study was conducted to determine if tillage and crop rotation affected subsurface drainage response to rainfall. An instrumentation system collected subsurface drain flow data from thirty-six, 0.4 ha plots during the 1993, 1994 and 1995 growing seasons. Response time, time-to-peak drain flow rate, drainage volume, peak drain flow rate and percent preferential flow were compared between two tillage systems (no-till and chisel plow) and two crop rotations (continuous corn and corn-soybean) for 23 drainage events over the three-year study. The influence of preferential flow was estimated for each drainage event using a hydrograph separation procedure based on subsurface drain flow rate changes.