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Belladonna/lhx2 is required for neural patterning and midline axon guidance in the zebrafish forebrain
Development (2006)
  • Anandita Seth
  • James Culverwell
  • Mitchell Walkowicz
  • Sabrina Toro
  • Jens M. Rick
  • Stephan C.F. Neuhauss
  • Zoltan M. Varga
  • Rolf O Karlstrom, University of Massachusetts - Amherst

Some of the earliest axon pathways to form in the vertebrate forebrain are established as commissural and retinal axons cross t he midline of the diencephalon and telencephalon. To better understand axon guidance in the forebrain, we characterized the zebrafish belladonna ( bel ) mutation, which disrupts commissural and retinal axon guidance in the forebrain. Using a positional cloning strategy, we determined that the bel locus encodes zebrafish Lhx2, a lim-homeodomain transcription factor expressed in the brain, eye and fin buds. We show that bel(lhx2) function is required for patterning in the ventral forebrain and eye, and that loss of bel function leads to alterations in regulatory gene expression, perturbations in axon guidance factors, and the absence of an optic chiasm and forebrain commissures. Our analysis reveals new roles for lhx2 in midline axon guidance, forebrain patterning and eye morphogenesis.

  • AC,
  • Chiasm,
  • Commissure,
  • Ephrin,
  • Gfap,
  • Glial bridge,
  • Morpholino,
  • Netrin,
  • POC,
  • Semaphorin,
  • Slit
Publication Date
February 15, 2006
Publisher Statement

DOI: 10.1242/dev.02244

The published version is located at

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Generic License.
Citation Information
Anandita Seth, James Culverwell, Mitchell Walkowicz, Sabrina Toro, et al.. "Belladonna/lhx2 is required for neural patterning and midline axon guidance in the zebrafish forebrain" Development Vol. 133 (2006)
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