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Lack of Artemisinin Resistance in Plasmodium falciparum in Uganda Based on Parasitological and Molecular Assays
Antimicrobial Agents and Chemotherapy
  • Roland A. Cooper, Department of Natural Sciences and Mathematics, Dominican University of California
  • Melissa D. Conrad, Department of Medicine, University of California, San Francisco, California, USA
  • Quentin D. Watson, Department of Integrative Biology, University of California, Berkeley
  • Stephanie J. Huezo, Department of Natural Sciences and Mathematics, Dominican University of California
  • Harriet Ninsiima, Infectious Diseases Research Collaboration, Kampala, Uganda
  • Patrick Tumwebaze, Infectious Diseases Research Collaboration, Kampala, Uganda
  • Samuel L. Nsobya, Infectious Diseases Research Collaboration, Kampala, Uganda
  • Philip J. Rosenthal, Department of Medicine, University of California, San Francisco
Document Type
Article
Publication Date
8-1-2015
Disciplines
Department
Natural Sciences and Mathematics
Abstract

We evaluated markers of artemisinin resistance in Plasmodium falciparum isolated in Kampala in 2014. By standard in vitro assays, all isolates were highly sensitive to dihydroartemisinin (DHA). By the ring-stage survival assay, after a 6-h DHA pulse, parasitemia was undetectable in 40 of 43 cultures at 72 h. Two of 53 isolates had nonsynonymous K13-propeller gene polymorphisms but did not have the mutations associated with resistance in Asia. Thus, we did not see evidence for artemisinin resistance in Uganda.

Rights

Copyright 2015 ©, American Society for Microbiology. All rights reserved.

Publisher Statement
Originally published as Cooper RA, Conrad MD, Watson QD, Huezo SJ, Ninsiima H, Tumwebaze P, Nsobya SL, Rosenthal PJ. 2015. Lack of artemisinin resistance in Plasmodium falciparum in Uganda based on parasitological and molecular assays. Antimicrob Agents Chemother 59:5061–5064.
Citation Information
Roland A. Cooper, Melissa D. Conrad, Quentin D. Watson, Stephanie J. Huezo, et al.. "Lack of Artemisinin Resistance in Plasmodium falciparum in Uganda Based on Parasitological and Molecular Assays" Antimicrobial Agents and Chemotherapy Vol. 59 Iss. 8 (2015) p. 5061 - 5064 ISSN: 0066-4804
Available at: http://works.bepress.com/roland_cooper/43/