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Article
Selection of Haploid Maize Kernels from Hybrid Kernels for Plant Breeding Using Near-Infrared Spectroscopy and SIMCA Analysis
Applied Spectroscopy
  • Roger W. Jones, Iowa State University
  • Tonu Reinot, Iowa State University
  • Ursula K. Frei, Iowa State University
  • Yichia Tseng, Iowa State University
  • Thomas Lübberstedt, Iowa State University
  • John F. McClelland, Iowa State University
Document Type
Article
Publication Date
4-1-2012
DOI
10.1366/11-06426
Abstract

Samples of haploid and hybrid seed from three different maize donor genotypes after maternal haploid induction were used to test the capability of automated near-infrared transmission spectroscopy to individually differentiate haploid from hybrid seeds. Using a two-step chemometric analysis in which the seeds were first classified according to genotype and then the haploid or hybrid status was determined proved to be the most successful approach. This approach allowed 11 of 13 haploid and 25 of 25 hybrid kernels to be correctly identified from a mixture that included seeds of all the genotypes.

Comments

This paper was published in Applied Spectroscopy 66 (2012): 447 and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: doi: 10.1366/11-06426.

Copyright Owner
Society for Applied Spectroscopy
Language
en
Date Available
2014-08-21
File Format
application/pdf
Citation Information
Roger W. Jones, Tonu Reinot, Ursula K. Frei, Yichia Tseng, et al.. "Selection of Haploid Maize Kernels from Hybrid Kernels for Plant Breeding Using Near-Infrared Spectroscopy and SIMCA Analysis" Applied Spectroscopy Vol. 66 Iss. 4 (2012) p. 447 - 450
Available at: http://works.bepress.com/roger_jones/1/