A rapid, sensitive and reliable gravity-flow alkaline elution assay was developed to detect DNA strand breaks in cultured Madin-Darby bovine kidney epithelial cells. Elution was completed within 2 h without the use of pumps. The system was validated by exposing the cells to X-irradiation (25–1500 R) which resulted in a significant dose dependent response (p<0.05) with excellent correlation (r=0.93). The assay reliably detected the DNA damage of seven genotoxic carcinogens. In general, the measured DNA damage was dose dependent and significantly different from control values for all genotoxic carcinogens tested. Six non-genotoxic compounds were tested and showed no detectable DNA damage.
Gravity-Flow Alkaline Elution: A Method to Rapidly Detect Carcinogen-Induced DNA Strand BreaksBiochemical and Biophysical Research Communications
Citation InformationHincks, J.R. and R.A. Coulombe, Jr. (1986). Gravity-flow alkaline elution: a method to rapidly detect carcinogen-induced DNA strand breaks. Biochem. Biophys. Res. Commun. 137:1006-1014.