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Article
Evaluation of Bone Regeneration in Implants Composed of Hollow HA Microspheres Loaded with Transforming Growth Factor Β1 in a Rat Calvarial Defect Model
Acta Biomaterialia
  • Hailuo Fu
  • M. N. Rahaman, Missouri University of Science and Technology
  • Roger F. Brown, Missouri University of Science and Technology
  • D. E. Day, Missouri University of Science and Technology
Editor(s)
Wagner, W. R.
Abstract

Implants that serve simultaneously as an osteoconductive matrix and as a device for local growth factor delivery may be required for optimal bone regeneration in some applications. In the present study, hollow hydroxyapatite (HA) microspheres (106-150μm) in the form of three-dimensional (3-D) scaffolds or individual (loose) microspheres were created using a glass conversion process. The capacity of the implants, with or without transforming growth factor β1 (TGF-β1), to regenerate bone in a rat calvarial defect model was compared. The 3-D scaffolds supported the proliferation and alkaline phosphatase activity of osteogenic MLO-A5 cells in vitro, showing their cytocompatibility. Release of TGF-β1 from the 3-D scaffolds into phosphate-buffered saline ceased after 2-3 days when ∼30% of the growth factor was released. Bone regeneration in the 3-D scaffolds and the individual microspheres increased with time from 6 to 12 weeks, but it was significantly higher (23%) in the individual microspheres than in the 3-D scaffolds (15%) after 12 weeks. Loading with TGF-β1 (5μg per defect) enhanced bone regeneration in the 3-D scaffolds and individual microspheres after 6 weeks, but had little effect after 12 weeks. 3-D scaffolds and individual microspheres with larger HA diameter (150-250μm) showed better ability to regenerate bone. Based on these results, implants composed of hollow HA microspheres show promising potential as an osteoconductive matrix for local growth factor delivery in bone regeneration.

Department(s)
Materials Science and Engineering
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2013 Elsevier, All rights reserved.
Publication Date
1-1-2013
Publication Date
01 Jan 2013
Citation Information
Hailuo Fu, M. N. Rahaman, Roger F. Brown and D. E. Day. "Evaluation of Bone Regeneration in Implants Composed of Hollow HA Microspheres Loaded with Transforming Growth Factor Β1 in a Rat Calvarial Defect Model" Acta Biomaterialia Vol. 9 Iss. 3 (2013) p. 5718 - 5727 ISSN: 1742-7061
Available at: http://works.bepress.com/roger-brown/15/