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Article
Induction of Apoptosis in Fibroblasts by IL-1ß-Converting Enzyme, a Mammalian Homolog of the C. elegans Cell Death Gene ced-3
Cell
  • Masayuki Miura, Massachusetts General Hospital
  • Hong Zhu, Massachusetts General Hospital
  • Rocco J. Rotello, Cedarville University
  • Erika A. Hartwieg, Massachusetts Institute of Technology
  • Junying Yuan, Massachusetts General Hospital
Document Type
Article
Publication Date
11-19-1993
DOI
http://dx.doi.org/10.1016/0092-8674(93)90486-a
PubMed ID
8242741
Abstract
The mammalian interleukin-1β-converting enzyme (ICE) has sequence similarity to the C. elegans cell death gene ced-3. We show here that overexpression of the murine ICE (mICE) gene or of the C. elegans ced-3 gene causes Rat-1 cells to undergo programmed cell death. Point mutations in a region homologous between mICE and CED-3 eliminate the ability of mICE and ced-3 to cause cell death. The cell death caused by mICE can be suppressed by overexpression of the crmA gene, a specific inhibitor of ICE, as well as by bcl-2, a mammalian oncogene that can act to prevent programmed cell death. Our results suggest that ICE may function during mammalian development to cause programmed cell death.
Keywords
  • Apoptosis,
  • fibroblasts,
  • IL-1ß converting enzyme
Citation Information
Masayuki Miura, Hong Zhu, Rocco J. Rotello, Erika A. Hartwieg, et al.. "Induction of Apoptosis in Fibroblasts by IL-1ß-Converting Enzyme, a Mammalian Homolog of the C. elegans Cell Death Gene ced-3" Cell Vol. 75 Iss. 4 (1993) p. 653 - 660
Available at: http://works.bepress.com/rocco_rotello/36/