Role of α-SNAP in Promoting Efficient Neurotransmission at the Crayfish Neuromuscular JunctionJournal of Neurophysiology (1999)
AbstractIn this manuscript, we address the role of the soluble N-ethylmaleimide sensitive factor attachment protein (α-SNAP) in synaptic transmission at the neuromuscular junction of the crayfish opener muscle. Immunochemcial methods confirm the presence of α-SNAP in these preparations and show that it is concentrated in the synaptic areas. Microinjection and electrophysiological studies show that α-SNAP causes an increase in neurotransmitter release yet does not significantly affect the kinetics. More specific quantal analysis, using focal, macropatch, synaptic current recordings, shows that α-SNAP increases transmitter release by increasing the probability of exocytosis but not the number of potential release sites. These data demonstrate that the role of α-SNAP is to increase the efficiency of neurotransmission by increasing the probability that a stimulus will result in neurotransmitter release. What this suggests is that α-SNAP is critical for the formation and maintenance of a “ready release” pool of synaptic vesicles.
- Neuromuscular Junction
Publication DateDecember 1, 1999
Citation InformationPing He, R. Chase Southard, Dong Chen, S. W. Whiteheart, et al.. "Role of α-SNAP in Promoting Efficient Neurotransmission at the Crayfish Neuromuscular Junction" Journal of Neurophysiology Vol. 82 Iss. 6 (1999)
Available at: http://works.bepress.com/robin_cooper/76/