Article
A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma
Sensors
Sponsor
This work was supported by the National Institutes of Health (RO1 EB002044)
Document Type
Article
Publication Date
5-1-2012
Subjects
- Fluorescence spectroscopy,
- Sulfur amino acids,
- Molecular probes
Disciplines
Abstract
A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols such as cysteine (Cys) and homocysteine (Hcy). The proposed method is precise with a relative standard deviation (R.S.D) lower than 6% (n = 3) and has been successfully applied to determine GSH in human plasma with recoveries between 99.2% and 102.3%.
DOI
10.3390/s120505940
Persistent Identifier
http://archives.pdx.edu/ds/psu/11114
Citation Information
Yixing, G., Xiaofeng, Y., Hakuna, L., Barve, A., Escobedo, J. O., Lowry, M., & Strongin, R. M. (2012). A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma. Sensors (14248220), 12(5), 5940-5950. doi:10.3390/s120505940
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