Skip to main content
Contribution to Book
Intrinsic Chemosensitivity of Individual Nucleus Tractus Solitarius (NTS) and Locus Coeruleus (LC) Neurons from Neonatal Rats
Integration in Respiratory Control: From Genes to Systems
  • Nicole L. Nichols
  • Lynn K. Hartzler, Wright State University - Main Campus
  • Susan C. Conrad
  • Jay B. Dean
  • Robert W. Putnam, Wright State University - Main Campus
Document Type
Book Chapter
Publication Date
1-1-2008
Abstract
Chemosensitive (CS) neurons are found in discrete brainstem regions, but whether the CS response of these neurons is due to intrinsic chemosensitivity of individual neurons or is mediated by changes in chemical and/or electrical synaptic input is largely unknown. We studied the effect of synaptic blockade (11.4 mM Mg2+/0.2 mM Ca2+) solution (SNB) and a gap junction uncoupling agent carbenoxolone (CAR—100 μM) on the response of neurons from two CS brainstem regions, the NTS and the LC. In NTS neurons, SNB decreased spontaneous firing rate (FR). We calculated the magnitude of the FR response to hypercapnic acidosis (HA; 15% CO2) using the Chemosensitivity Index (CI). The percentage of NTS neurons activated and CI were the same in the absence and presence of SNB. Blocking gap junctions with CAR did not significantly alter spontaneous FR. CAR did not alter the CI in NTS neurons and resulted in a small decrease in the percentage of activated neurons, which was most evident in NTS neurons from rats younger than postnatal day 10. In LC neurons, SNB resulted in an increase in spontaneous FR. As with NTS neurons, SNB did not alter the percentage of activated neurons or the CI in LC neurons. CAR resulted in a small increase in spontaneous FR in LC neurons.
DOI
10.1007/978-0-387-73693-8_61
Citation Information
Nicole L. Nichols, Lynn K. Hartzler, Susan C. Conrad, Jay B. Dean, et al.. "Intrinsic Chemosensitivity of Individual Nucleus Tractus Solitarius (NTS) and Locus Coeruleus (LC) Neurons from Neonatal Rats" Integration in Respiratory Control: From Genes to Systems Vol. 605 (2008) p. 348 - 352 ISSN: 0387736921
Available at: http://works.bepress.com/robert_putnam/73/