Design and fabrication of a COP-based microfluidic chip: Chronoamperometric detection of Troponin TAustralian Institute for Innovative Materials - Papers
AbstractThis work demonstrates the design and fabrication of an all cyclo-olefin polymer based microfluidic device capable of capturing magnetic beads and performing electrochemical detection in a series of gold electrodes. The size of chip is of a microscope slide and features six independent measuring cells formultianalyte detection purposes. The aim of this work is to show that rapid prototyping techniques can be instrumental in the development of novel bioassays, particularly in clinical diagnosis applications. We show the successful determination of troponin-T, a cardiac disease marker, in the clinically relevant range of 0.05–1.0 ng/mL. This methodology achieves a detection limit of 0.017 ng/mL in PBS solutions, and is capable of detecting less than 1 ng/mL in a 1:50 human serum dilution.
Citation InformationLlibertat Abad, Francisco Javier Del Campo, Francesc Xavier Munoz, Luis J Fernandez, et al.. "Design and fabrication of a COP-based microfluidic chip: Chronoamperometric detection of Troponin T" (2012) p. 3187 - 3194
Available at: http://works.bepress.com/robert_gorkin/8/