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Design and fabrication of a COP-based microfluidic chip: Chronoamperometric detection of Troponin T
Australian Institute for Innovative Materials - Papers
  • Llibertat Abad, Universitat Auto´ noma de Barcelona
  • Francisco Javier Del Campo, Universitat Auto´ noma de Barcelona
  • Francesc Xavier Munoz, Universitat Auto´ noma de Barcelona
  • Luis J Fernandez, University of Zaragoza
  • Daniel Calavia, Alphasip S. L., Zaragoza, Spain
  • Gloria Colom, Applied Molecular Receptors Group (AMRg), IQAC-CSIC, Barcelona
  • Juan P Salvador, Applied Molecular Receptors Group (AMRg), IQAC-CSIC, Barcelona
  • Maria Pilar Marco, Applied Molecular Receptors Group (AMRg), IQAC-CSIC, Barcelona
  • Vanessa Escamilla-Gomez, Universidad Complutense de Madrid
  • Berta Esteban-Fernandez De Avila, Universidad Complutense de Madrid
  • Susana Campuzano, Universidad Complutense de Madrid
  • Maria Pedrero, Universidad Complutense de Madrid
  • Jose M Pingarron, Universidad Complutense de Madrid
  • Neus Godino, Dublin City University
  • Robert Gorkin III, Dublin City University
  • Jens Ducree, Dublin City University
RIS ID
78554
Publication Date
1-1-2012
Publication Details

Abad, L., Del Campo, F., Munoz, F., Fernandez, L., Calavia, D., Colom, G., Salvador, J., Marco, M., Escamilla-Gomez, V., De Avila, B., Campuzano, S., Pedrero, M., Pingarron, J., Godino, N., Gorkin III, R. & Ducree, J. (2012). Design and fabrication of a COP-based microfluidic chip: Chronoamperometric detection of Troponin T. Electrophoresis, 33 (21), 3187-3194.

Abstract

This work demonstrates the design and fabrication of an all cyclo-olefin polymer based microfluidic device capable of capturing magnetic beads and performing electrochemical detection in a series of gold electrodes. The size of chip is of a microscope slide and features six independent measuring cells formultianalyte detection purposes. The aim of this work is to show that rapid prototyping techniques can be instrumental in the development of novel bioassays, particularly in clinical diagnosis applications. We show the successful determination of troponin-T, a cardiac disease marker, in the clinically relevant range of 0.05–1.0 ng/mL. This methodology achieves a detection limit of 0.017 ng/mL in PBS solutions, and is capable of detecting less than 1 ng/mL in a 1:50 human serum dilution.

Citation Information
Llibertat Abad, Francisco Javier Del Campo, Francesc Xavier Munoz, Luis J Fernandez, et al.. "Design and fabrication of a COP-based microfluidic chip: Chronoamperometric detection of Troponin T" (2012) p. 3187 - 3194
Available at: http://works.bepress.com/robert_gorkin/8/