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Fluidic automation of nitrate and nitrite bioassays in whole blood by dissolvable-film based centrifugo-pneumatic actuation
Australian Institute for Innovative Materials - Papers
  • Charles E Nwankire, Dublin City University
  • Di-Sien S Chan, Dublin City University
  • Jennifer Gaughran, Dublin City University
  • Robert Burger, Dublin City University
  • Robert A Gorkin III, University of Wollongong
  • Jens Ducree, Dublin City University
RIS ID
82343
Publication Date
1-1-2013
Publication Details

Nwankire, C. E., Chan, D. S., Gaughran, J., Burger, R., Gorkin III, R. A. and Ducree, J. (2013). Fluidic automation of nitrate and nitrite bioassays in whole blood by dissolvable-film based centrifugo-pneumatic actuation. Sensors (Switzerland), 13 (9), 11336-11349.

Abstract

This paper demonstrates the full centrifugal microfluidic integration and automation of all liquid handling steps of a 7-step fluorescence-linked immunosorbent assay (FLISA) for quantifying nitrate and nitrite levels in whole blood within about 15 min. The assay protocol encompasses the extraction of metered plasma, the controlled release of sample and reagents (enzymes, co-factors and fluorescent labels), and incubation and detection steps. Flow control is implemented by a rotationally actuated dissolvable film (DF) valving scheme. In the valves, the burst pressure is primarily determined by the radial position, geometry and volume of the valve chamber and its inlet channel and can thus be individually tuned over an extraordinarily wide range of equivalent spin rates between 1,000 RPM and 5,500 RPM. Furthermore, the vapour barrier properties of the DF valves are investigated in this paper in order to further show the potential for commercially relevant on-board storage of liquid reagents during shelf-life of bioanalytical, ready-to-use discs.

Citation Information
Charles E Nwankire, Di-Sien S Chan, Jennifer Gaughran, Robert Burger, et al.. "Fluidic automation of nitrate and nitrite bioassays in whole blood by dissolvable-film based centrifugo-pneumatic actuation" (2013) p. 11336 - 11349
Available at: http://works.bepress.com/robert_gorkin/15/