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Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF
Journal of Translational Medicine
  • Harold Brem, New York University School of Medicine
  • Michael S. Golinko, New York University School of Medicine
  • Olivera Stojadinovic, Cornell University
  • Arber Kodra, New York University School of Medicine
  • Robert F. Diegelmann, Virginia Commonwealth University
  • Sasa Vukelic, Cornell University
  • Hyacinth Entero, Ross University School of Medicine
  • Donald L. Coppock, Coriell Institute for Medical Research
  • Marjana Tomic-Canic, Cornell University
Document Type
Article
Original Publication Date
2008
DOI of Original Publication
10.1186/1479-5876-6-75
Comments

Originally published at http://dx.doi.org/10.1186/1479-5876-6-75

Date of Submission
September 2014
Abstract
Background Multiple physiologic impairments are responsible for chronic wounds. A cell line grown which retains its phenotype from patient wounds would provide means of testing new therapies. Clinical information on patients from whom cells were grown can provide insights into mechanisms of specific disease such as diabetes or biological processes such as aging. The objective of this study was 1) To culture human cells derived from patients with chronic wounds and to test the effects of putative therapies, Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) on these cells. 2) To describe a methodology to create fibroblast cell lines from patients with chronic wounds. Methods Patient biopsies were obtained from 3 distinct locations on venous ulcers. Fibroblasts derived from different wound locations were tested for their migration capacities without stimulators and in response to GM-CSF. Another portion of the patient biopsy was used to develop primary fibroblast cultures after rigorous passage and antimicrobial testing. Results Fibroblasts from the non-healing edge had almost no migration capacity, wound base fibroblasts were intermediate, and fibroblasts derived from the healing edge had a capacity to migrate similar to healthy, normal, primary dermal fibroblasts. Non-healing edge fibroblasts did not respond to GM-CSF. Six fibroblast cell lines are currently available at the National Institute on Aging (NIA) Cell Repository. Conclusion We conclude that primary cells from chronic ulcers can be established in culture and that they maintain their in vivo phenotype. These cells can be utilized for evaluating the effects of wound healing stimulators in vitro.
Rights
© 2008 Brem et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Is Part Of
VCU Biochemistry and Molecular Biology Publications
Citation Information
Harold Brem, Michael S. Golinko, Olivera Stojadinovic, Arber Kodra, et al.. "Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF" Journal of Translational Medicine Vol. 6 (2008)
Available at: http://works.bepress.com/robert_diegelmann/2/