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Tuning caged calcium: Photolabile analogues of EGTA with improved optical and chelation properties
Cell calcium
  • G. C. R. Ellis-Davies
  • Robert J. Barsotti, Philadelphia College of Osteopathic Medicine
Document Type
Article
Publication Date
1-1-2006
Disciplines
Abstract
The physico-chemical properties of several Ca2+-selective, photolabile chelators are described. These molecules have been developed as part of an effort to produce a caged Ca2+ that improved upon the Ca2+ chelation properties and light absorption capability of nitrophenyl-EGTA (NP-EGTA). Four dimethoxy-ortho-nitrophenyl derivatives of EGTA (called DMNPE-1 through -4), and one analogue of EGTA (DMNPE-5) have been characterized, each of which is bisected upon irradiation. One of these cages has a higher affinity than NP-EGTA: DMNPE-4 has a Kd for Ca2+ of 48 nm at pH 7.2 (19 nM at pH 7.4). Furthermore, this cage has a large extinction coefficient of 5120 M-1 cm-1 at 350 nm (cf. 975 M-1 cm-1 for NP-EGTA). The other physico-chemical properties of DMNPE-4 are: quantum yield of photolysis of 0.09; bipasic Ca2+ release kinetics (70% released with a rate of about 48,000 s-1 and 30% at 1.5 s-1) and photoproducts that bind Ca2+ with very low affinity (Kd in the range of 2mM, pH 7.2), hence most of the bound Ca2+ is released rapidly and efficiently upon photolysis. Thus, DMNPE-4 has a unique combination of properties that make it an extremely effective Ca2+ cage. © 2005 Elsevier Ltd. All rights reserved.
Comments

This article was published in Cell calcium, Volume 39, Issue 1, Pages 75-83.

The published version is available at http://dx.doi.org/10.1016/j.ceca.2005.10.003.

Copyright © 2006 Elsevier.

Citation Information
G. C. R. Ellis-Davies and Robert J. Barsotti. "Tuning caged calcium: Photolabile analogues of EGTA with improved optical and chelation properties" Cell calcium Vol. 39 Iss. 1 (2006) p. 75 - 83
Available at: http://works.bepress.com/robert_barsotti/25/