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Article
Diazepam, y-aminobutyric acid, and progesterone open K+ channels in myocytes from coronary arteries
European journal of pharmacology
  • Maryanne K. Jacob
  • Richard E. White, Philadelphia College of Osteopathic Medicine
Document Type
Article
Publication Date
1-1-2000
Disciplines
Abstract
Benzodiazepines enhance coronary blood flow and lower blood pressure, but the cellular basis of this action remains unclear. The present study now demonstrates a direct effect of diazepam, y-aminobutyric acid (GABA), and progesterone on the large conductance, Ca2+- and voltage-activated K+ channel (BK(Ca)) in single myocytes isolated from porcine coronary arteries. These GABA receptor agonists significantly increased whole-cell (perforated patch) K+ currents and stimulated the activity of single BK(Ca) channels in cell-attached patches dramatically. This effect is not mediated via cyclic AMP or cyclic GMP, but involves stimulation of Ca2+ influx in response to activation of a bicuculline-sensitive GABA(A)-like receptor. We propose that localized, subsarcolemmal increases in Ca2+ levels open BK(Ca) channels, thereby promoting K+ efflux, membrane repolarization, and coronary relaxation. This transduction pathway can now account, at least in part, for the direct vasodilatory effects of diazepam, progesterone, and GABA.
Comments

This article was published in European journal of pharmacology, Volume 403, Issue 3, Pages 209-219.

The published version is available at http://dx.doi.org/10.1016/S0014-2999(00)00598-7.

Copyright © 2000 Elsevier.

Citation Information
Maryanne K. Jacob and Richard E. White. "Diazepam, y-aminobutyric acid, and progesterone open K+ channels in myocytes from coronary arteries" European journal of pharmacology Vol. 403 Iss. 3 (2000) p. 209 - 219
Available at: http://works.bepress.com/richard_white/46/