Skip to main content
Article
Kinetic and Spectroscopic Analysis of the Catalytic Role of H79 in the Methionine Aminopeptidase from Escherichia coli
Biochemistry
  • Sarah J. Watterson, Utah State University
  • Sanghamitra Mitra, Utah State University
  • Sabina I. Swierczek, Utah State University
  • Brian Bennett, Marquette University
  • Richard C. Holz, Marquette University
Document Type
Article
Language
eng
Format of Original
9 p.
Publication Date
11-1-2008
Publisher
American Chemical Society
Original Item ID
doi: 10.1021/bi801499g
Disciplines
Abstract
To gain insight into the role of the strictly conserved histidine residue, H79, in the reaction mechanism of the methionyl aminopeptidase from Escherichia coli (EcMetAP-I), the H79A mutated enzyme was prepared. Co(II)-loaded H79A exhibits an overall >7000-fold decrease in specific activity. The almost complete loss of activity is primarily due to a >6000-fold decrease in kcat. Interestingly, the Km value obtained for Co(II)-loaded H79A was approximately half the value observed for wild-type (WT) EcMetAP-I. Consequently, kcat/Km values decreased only 3000-fold. On the other hand, the observed specific activity of Mn(II)-loaded H79A EcMetAP-I decreased by ∼2.6-fold while kcat decreased by ∼3.5-fold. The observed Km value for Mn(II)-loaded H79A EcMetAP-I was ∼1.4-fold larger than that observed for WT EcMetAP-I, resulting in a kcat/Km value that is lower by ∼3.4-fold. Metal binding, UV−vis, and EPR data indicate that the active site is unperturbed by mutation of H79, as suggested by X-ray crystallographic data. Kinetic isotope data indicate that H79 does not transfer a proton to the newly forming amine since a single proton is transferred in the transition state for both the WT and H79A EcMetAP-I enzymes. Therefore, H79 functions to position the substrate by hydrogen bonding to either the amine group of the peptide linkage or a backbone carbonyl group. Together, these data provide new insight into the catalytic mechanism of EcMetAP-I.
Comments

Biochemistry, Vol. 47, No. 45 (November 2008): 11885–11893. DOI.

Brian Bennett was affiliated with Medical College of Wisconsin at the time of publication.

Richard C. Holz was affiliated with Loyola University Chicago at the time of publication.

Citation Information
Sarah J. Watterson, Sanghamitra Mitra, Sabina I. Swierczek, Brian Bennett, et al.. "Kinetic and Spectroscopic Analysis of the Catalytic Role of H79 in the Methionine Aminopeptidase from Escherichia coli" Biochemistry (2008) ISSN: 0006-2960
Available at: http://works.bepress.com/richard_holz/49/