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Immobilization of Motile Bacterial Cells via Dip-pen Nanolithography
Nanotechnology
  • Dorjderem Nyamjav, Loyola University Chicago
  • Sergey Rozhok, Northwestern University
  • Richard C. Holz, Marquette University
Document Type
Article
Publication Date
1-1-2010
Disciplines
Abstract
A strategy to bind bacterial cells to surfaces in a directed fashion via dip-pen nanolithography (DPN) is presented. Cellular attachment to pre-designed DPN generated microarrays was found to be dependent on the shape and size of the surface feature. While this observation is likely due in part to a dense, well formed mercaptohexadecanoic acid (MHA) monolayer generated via DPN, it may also simply be due to the physical shape of the surface structure. Motile Pseudomonas aeruginosa bacterial cells were observed to bind to DPN generated mercaptohexadecanoic acid/poly-L-lysine (MHA/PLL) line patterns, 'blocks' made up of eight lines with 100 nm spacings, with ~ 80% occupancy. Cellular binding to these 'block' surface structures occurs via an electrostatic interaction between negatively charged groups on the bacterial cell surface and positively charged poly-L-lysine (PLL) assemblies. These data indicate that these DPN generated 'block' surface structures provide a promising footprint for the attachment of motile bacterial cells that may find utility in cell based biosensors or single cell studies.
Comments

Nanotechnology. Vol. 21, No. 23 (2010): 235105. DOI.

Richard C. Holz was affiliated with Loyola University-Chicago at the time of publication.

Citation Information
Dorjderem Nyamjav, Sergey Rozhok and Richard C. Holz. "Immobilization of Motile Bacterial Cells via Dip-pen Nanolithography" Nanotechnology (2010) ISSN: 1361-6528
Available at: http://works.bepress.com/richard_holz/3/