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Article
Mutation of H63 and its Catalytic Affect on the Methionine Aminopeptidase from Escherichia coli
Biochimica et Biophysica Acta: Proteins and Proteomics
  • Sanghamitra Mitra, Loyola University Chicago
  • Brian Bennett, Medical College of Wisconsin
  • Richard C. Holz, Marquette University
Document Type
Article
Language
eng
Format of Original
7 p.
Publication Date
1-1-2009
Publisher
Elsevier
Original Item ID
DOI: 10.1016/j.bbapap.2008.09.012, PubMed Central: PMC2674292
Disciplines
Abstract
In order to gain insight into the mechanistic role of a flexible exterior loop near the active site, made up of Y62, H63, G64, and Y65, that has been proposed to play an important role in substrate binding and recognition in the methionyl aminopeptidase from Escherichia coli (EcMetAP-I), the H63A enzyme was prepared. Mutation of H63 to alanine does not affect the ability of the enzyme to bind divalent metal ions. The specific activity of H63A EcMetAP-I was determined using four different substrates of varying lengths, namely, l-Met-p-NA, MAS, MGMM and MSSHRWDW. For the smallest/shortest substrate (l-Met-p-NA) the specific activity decreased nearly seven fold but as the peptide length increased, the specific activity also increased and became comparable to WT EcMetAP-I. This decrease in specific activity is primarily due to a decrease in the observed kcat values, which decreases nearly sixty-fold for l-Met-p-NA while only a four-fold decrease is observed for the tri- and tetra-peptide substrates. Interestingly, no change in kcat was observed when the octa-peptide MSSHRWDW was used as a substrate. These data suggest that H63 affects the hydrolysis of small peptide substrates whereas large peptides can overcome the observed loss in binding energy, as predicted from Km values, by additional hydrophilic and hydrophobic interactions.
Comments

Accepted version. Biochimica et Biophysica Acta: Proteins and Proteomics. Vol. 1794, No. 1 (January 2009): 137-143. DOI. © Elsevier, 2008. Used with permission

NOTICE: this is the author’s version of a work that was accepted for publication in Biochimica et Biophysica Acta: Proteins and Proteomics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochimica et Biophysica Acta: Proteins and Proteomics, VOL 1794, ISSUE 1, January 2009, DOI.

Richard C. Holz was affiliated with Loyola University-Chicago at the time of publication.

Brian Bennett was affiliated with The Medical College of Wisconsin at the time of publication.

Citation Information
Sanghamitra Mitra, Brian Bennett and Richard C. Holz. "Mutation of H63 and its Catalytic Affect on the Methionine Aminopeptidase from Escherichia coli" Biochimica et Biophysica Acta: Proteins and Proteomics (2009) ISSN: 0006-3002
Available at: http://works.bepress.com/richard_holz/13/