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Article
A helitron-like transposon superfamily from lepidoptera disrupts (GAAA)(n) microsatellites and is responsible for flanking sequence similarity within a microsatellite family
Journal of Molecular Evolution
  • Brad S. Coates, Iowa State University
  • Douglas V. Sumerford, United States Department of Agriculture
  • Richard L. Hellmich, Iowa State University
  • Leslie C. Lewis, United States Department of Agriculture
Document Type
Article
Publication Date
3-1-2010
DOI
10.1007/s00239-010-9330-6
Abstract
Transposable elements (TEs) are mobile DNA regions that alter host genome structure and gene expression. A novel 588 bp non-autonomous high copy number TE in the Ostrinia nubilalis genome has features in common with miniature inverted-repeat transposable elements (MITEs): high A + T content (62.3%), lack of internal protein coding sequence, and secondary structure consisting of subterminal inverted repeats (SIRs). The O. nubilalis TE has inserted at (GAAA)n microsatellite loci, and was named the microsatellite-associated interspersed nuclear element ( MINE-1). Non-autonomous MINE-1 superfamily members also were identified downstream of (GAAA)n microsatellites within Bombyx mori and Pectinophora gossypiella genomes. Of 316 (GAAA)n microsatellites from the B. mori whole genome sequence, 201 (63.6%) have associated autonomous or non-autonomous MINE-1 elements. Autonomous B. mori MINE-1s a encode a helicase and endonuclease domain RepHel-like protein (BMHELp1) indicating their classification as Helitron-like transposons and were renamed Helitron1_BM. Transposition of MINE-1 members in Lepidoptera has resulted in the disruption of (GAAA)n microsatellite loci, has impacted the application of microsatellite-based genetic markers, and suggests genome sequence that flanks TT/AA dinucleotides may be required for target site recognition by RepHel endonuclease domains.
Comments

This article is from Journal of Molecular Evolution; 70 (2010); 275-288; doi: 10.1007/s00239-010-9330-6

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
Date Available
2014-08-28
File Format
application/pdf
Citation Information
Brad S. Coates, Douglas V. Sumerford, Richard L. Hellmich and Leslie C. Lewis. "A helitron-like transposon superfamily from lepidoptera disrupts (GAAA)(n) microsatellites and is responsible for flanking sequence similarity within a microsatellite family" Journal of Molecular Evolution Vol. 70 Iss. 3 (2010) p. 275 - 288
Available at: http://works.bepress.com/richard_hellmich/90/