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Article
A beta-1,3-galactosyltransferase and brainiac/bre5 homolog expressed in the midgut did not contribute to a Cry1Ab toxin resistance trait in Ostrinia nubilalis
Insect Biochemistry and Molecular Biology
  • Brad S. Coates, Iowa State University
  • Douglas V. Sumerford, United States Department of Agriculture
  • Richard L. Hellmich, Iowa State University
  • Leslie C. Lewis, Iowa State University
Document Type
Article
Disciplines
Publication Date
1-1-2007
DOI
10.1016/j.ibmb.2006.12.008
Abstract

Post-translational glycosylation of midgut epithelial protein and lipid receptors may be required prior to binding of activated Bacillus thuringiensis (Bt) Cry toxins. A 931bp cDNA encoding a putative 297-residue beta-1,3-galactosyltransferase (beta3GalT5) was cloned from larval Ostrinia nubilalis midgut tissue, and showed homology to Drosophila brainiac (brn) and Caenorhabditis elegans bre5 proteins. Single nucleotide polymorphisms (SNPs) were detected in coding and promoter regions of O. nubilalis beta3GalT5 (Onb3GalT5), of which 3 of 31 CDS SNPs were non-synonymous. SNPs within HaeIII and MspI recognition sites were confirmed by PCR-RFLP, and are Mendelian inherited. Analysis of F(2) pedigrees suggested an Onb3GalT5 SNP C660 fixed within a Cry1Ab-resistant colony was not correlated with Cry1Ab resistance traits, as measured by higher larval O. nubilalis weights when fed toxin-containing diet.

Comments

This article is from Insect Biochemistry and Molecular Biology; 37 (2007); 346-355; doi: 10.1016/j.ibmb.2006.12.008

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
Date Available
2014-08-27
File Format
application/pdf
Citation Information
Brad S. Coates, Douglas V. Sumerford, Richard L. Hellmich and Leslie C. Lewis. "A beta-1,3-galactosyltransferase and brainiac/bre5 homolog expressed in the midgut did not contribute to a Cry1Ab toxin resistance trait in Ostrinia nubilalis" Insect Biochemistry and Molecular Biology Vol. 37 Iss. 4 (2007) p. 346 - 355
Available at: http://works.bepress.com/richard_hellmich/89/