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Transcriptome sequencing, and rapid development and application of SNP markers for the legume pod borer Maruca vitrata (Lepidoptera: Crambidae)
PLOS ONE
  • Venu M. Margam, Purdue University
  • Brad S. Coates, United States Department of Agriculture
  • Darrell O. Bayles, United States Department of Agriculture
  • Richard L. Hellmich, Iowa State University
  • Tolulope Agunbiade, University of Illinois at Urbana-Champaign
  • Manfredo J. Seufferheld, University of Illinois at Urbana-Champaign
  • Weilin Sun, University of Illinois at Urbana-Champaign
  • Jeremy A. Kroemer, United States Department of Agriculture
  • Malick N. Ba, Institut de l'Environment et de Recherches Agricoles (INERA)
  • Clementine L. Binso-Dabire, Institut de l'Environment et de Recherches Agricoles (INERA)
  • Ibrahim Baoua, Institut National de Recherche Agronomique du Niger
  • Mohammed F. Ishiyaku, Institute for Agricultural Research
  • Fernando G. Covas, University of Puerto Rico - Mayaguez
  • Ramasamy Srinivasan, AVRDC-The World Vegetable Center
  • Joel Armstrong, The Commonwealth of Scientific and Industrial Research Organization
  • Larry L. Murdock, Purdue University
  • Barry R. Pittendrigh, University of Illinois at Urbana-Champaign
Document Type
Article
Publication Date
7-1-2011
DOI
10.1371/journal.pone.0021388
Abstract
The legume pod borer, Maruca vitrata (Lepidoptera: Crambidae), is an insect pest species of crops grown by subsistence farmers in tropical regions of Africa. We present the de novo assembly of 3729 contigs from 454- and Sanger-derived sequencing reads for midgut, salivary, and whole adult tissues of this non-model species. Functional annotation predicted that 1320 M. vitrata protein coding genes are present, of which 631 have orthologs within the Bombyx mori gene model. A homology-based analysis assigned M. vitrata genes into a group of paralogs, but these were subsequently partitioned into putative orthologs following phylogenetic analyses. Following sequence quality filtering, a total of 1542 putative single nucleotide polymorphisms (SNPs) were predicted within M. vitrata contig assemblies. Seventy one of 1078 designed molecular genetic markers were used to screen M. vitrata samples from five collection sites in West Africa. Population substructure may be present with significant implications in the insect resistance management recommendations pertaining to the release of biological control agents or transgenic cowpea that express Bacillus thuringiensis crystal toxins. Mutation data derived from transcriptome sequencing is an expeditious and economical source for genetic markers that allow evaluation of ecological differentiation.
Comments

This article is from PLOS ONE; 6 (2011); e21388; doi: 10.1371/journal.pone.0021388

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Citation Information
Venu M. Margam, Brad S. Coates, Darrell O. Bayles, Richard L. Hellmich, et al.. "Transcriptome sequencing, and rapid development and application of SNP markers for the legume pod borer Maruca vitrata (Lepidoptera: Crambidae)" PLOS ONE Vol. 6 Iss. 7 (2011) p. e21388
Available at: http://works.bepress.com/richard_hellmich/85/