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Article
Transcript analysis and comparative evaluation of shaker and slowmo gene homologues from the European corn borer, Ostrinia nubilalis
Insect Molecular Biology
  • Jeremy A. Kroemer, United States Department of Agriculture
  • Tyasning Nusawardani, Iowa State University
  • Michael Allen Rausch, Iowa State University
  • Susan E. Moser, United States Department of Agriculture
  • Richard L. Hellmich, Iowa State University
Document Type
Article
Disciplines
Publication Date
8-1-2011
DOI
10.1111/j.1365-2583.2011.01080.x
Abstract

The movement and dispersal of larval Lepidoptera impact their survival and distribution within the natural landscape. Homologues of the Drosophila behaviour-linked genes shaker (shkr) and slowmo (slmo) were identified from Ostrinia nubilalis (Lepidoptera: Crambidae). Onshkr was isolated as a 1610-nucleotide (nt) constitutively expressed transcript encoding a membrane-localized 469-amino-acid (aa) protein with a conserved tetramerization domain and the six-domain architecture necessary for the molecule to fold into an active K(+) channel. Three expressed splice variants of 682, 970 and 1604 nt were identified for the Onslmo gene, and encode predicted 141 and 228 aa proteins with a conserved protein of relevant evolutionary and lymphoid interest (PRELI) domain that may function in mitochondrial protein sorting and perinuclear protein localization. Onshkr and Onslmo protein sequences aligned within monophyletic lepidopteran groups.

Comments

This article is from Insect Molecular Biology; 20 (2011); 493-506; doi: 10.1111/j.1365-2583.2011.01080.x

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
Date Available
2014-09-02
File Format
application/pdf
Citation Information
Jeremy A. Kroemer, Tyasning Nusawardani, Michael Allen Rausch, Susan E. Moser, et al.. "Transcript analysis and comparative evaluation of shaker and slowmo gene homologues from the European corn borer, Ostrinia nubilalis" Insect Molecular Biology Vol. 20 Iss. 4 (2011) p. 493 - 506
Available at: http://works.bepress.com/richard_hellmich/78/