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Article
Sequence variation in the cadherin gene of Ostrinia nubilalis: a tool for field monitoring
Insect Biochemistry and Molecular Biology
  • Brad S. Coates, United States Department of Agriculture
  • Douglas V. Sumerford, United States Department of Agriculture
  • Richard L. Hellmich, Iowa State University
  • Leslie C. Lewis, Iowa State University
Document Type
Article
Publication Date
10-1-2004
DOI
10.1016/j.ibmb.2004.10.008
Abstract

Toxin-binding proteins of insect midgut epithelial cells are associated with insect resistance to Bacillus thuringiensis (Bt) Cry toxins. A 5378 nt cDNA encoding a 1717 amino acid putative midgut cadherin-like glycoprotein and candidate Cry1Ab toxin-binding protein was characterized from Ostrinia nubilalis. Intraspecific alignment of partial O. nubilalis cadherin gene sequences identified variance within proposed Cry1A toxin binding region 2 (TBR2), 1328IPLQTSILVVT[I/V] N1340, and flanking Cry1A toxin binding region 1 (TBR1), 861DIEIEIIDTNN871. DNA sequence and PCR-RFLP detected single nucleotide polymorphism between cadherin alleles, and pedigree analysis demonstrated Mendelian inheritance. A population sample from Mead, Nebraska showed allelic polymorphism. These assays may be useful for linkage mapping and field surveillance of wild populations and of O. nubilalis.

Comments

This article is from Insect Biochemistry and Molecular Biology; 35 (2005); 129-139; doi: 10.1016/j.ibmb.2004.10.008

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
Date Available
2014-08-26
File Format
application/pdf
Citation Information
Brad S. Coates, Douglas V. Sumerford, Richard L. Hellmich and Leslie C. Lewis. "Sequence variation in the cadherin gene of Ostrinia nubilalis: a tool for field monitoring" Insect Biochemistry and Molecular Biology Vol. 35 Iss. 2 (2004) p. 129 - 139
Available at: http://works.bepress.com/richard_hellmich/71/