Skip to main content
Article
Mining an Ostrinia nubilalis midgut expressed sequence tag (EST) library for candidate genes and single nucleotide polymorphisms (SNPs)
Insect Molecular Biology
  • Brad S. Coates, United States Department of Agriculture
  • Douglas V. Sumerford, United States Department of Agriculture
  • Richard L. Hellmich, Iowa State University
  • Leslie C. Lewis, Iowa State University
Document Type
Article
Publication Date
12-1-2008
DOI
10.1111/j.1365-2583.2008.00833.x
Abstract

Genes expressed in lepidopteran midgut tissues are involved in digestion and Bacillus thuringiensis (Bt) toxin resistance traits. Five hundred and thirty five unique transcripts were annotated from 1745 high quality O. nubilalis larval midgut expressed sequence tags (ESTs). Full-length cDNA sequence of 12 putative serine proteinase genes and 3 partial O. nubilalis aminopeptidase N protein genes, apn1, apn3, and apn4, were obtained, and genes may have roles in plant feeding and Bt toxin resistance traits of Ostrinia larvae. The EST library was not normalized and insert frequencies reflect transcript levels under the initial treatment conditions and redundancy of inserts from highly expressed transcripts allowed prediction of putative single nucleotide polymorphisms (SNPs). Ten di-, tri- or tetranucleotide repeat unit microsatellite loci were identified, and minisatellite repeats were observed within the C-termini of two encoded serine proteinases. Molecular markers showed polymorphism at 28 SNP loci and one microsatellite locus, and Mendelian inheritance indicated that markers were applicable to genome mapping applications. This O. nubilalis larval midgut EST collection is a resource for gene discovery, expression information, and allelic variation for use in genetic marker development.

Comments

This article is from Insect Molecular Biology; 17 (2008); 607-620; doi: 10.1111/j.1365-2583.2008.00833.x

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
Date Available
2014-08-27
File Format
application/pdf
Citation Information
Brad S. Coates, Douglas V. Sumerford, Richard L. Hellmich and Leslie C. Lewis. "Mining an Ostrinia nubilalis midgut expressed sequence tag (EST) library for candidate genes and single nucleotide polymorphisms (SNPs)" Insect Molecular Biology Vol. 17 Iss. 6 (2008) p. 607 - 620
Available at: http://works.bepress.com/richard_hellmich/64/