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Effects on Monarch Butterfly Larvae (Lepidoptera: Danaidae) After Continuous Exposure to Cry1Ab-Expressing Corn During Anthesis
Environmental Entomology
  • Galen P. Dively, University of Maryland - College Park
  • Robyn Rose, University of Guelph
  • Mark K. Sears, University of Guelph
  • Richard L. Hellmich, Iowa State University
  • Diane E. Stanley-Horn, Pennsylvania State University - Main Campus
  • Dennis D. Calvin, Pennsylvania State University - Main Campus
  • Joseph M. Russo, ZedX - Bellefont, PA
  • Patricia L. Anderson, Iowa State University
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Effects on monarch butterfly, Danaus plexippus L., after continuous exposure of larvae to natural deposits of Bacillus thuringiensis (Bt) and non-Bt pollen on milkweed, were measured in five studies. First instars were exposed at 3–4 and 6–7 d after initial anthesis, either directly on milkweed plants in commercial cornfields or in the laboratory on leaves collected from milkweeds in corn plots. Pollen exposure levels ranging from 122 to 188 grains/cm2/d were similar to within-field levels that monarch butterfly populations might experience in the general population of cornfields. Results indicate that 23.7% fewer larvae exposed to these levels of Bt pollen during anthesis reached the adult stage. A risk assessment procedure used previously was updated with a simulation model estimating the proportion of second-generation monarch butterflies affected. When considered over the entire range of the Corn Belt, which represents only 50% of the breeding population, the risk to monarch butterfly larvae associated with long-term exposure to Bt corn pollen is 0.6% additional mortality. Exposure also prolonged the developmental time of larvae by 1.8 d and reduced the weights of both pupae and adults by 5.5%. The sex ratio and wing length of adults were unaffected. The ecological significance of these sublethal effects is discussed relative to generation mortality and adult performance.

This article is from Environmental Entomology; 33 (2004); 1116-1125; doi: 10.1603/0046-225X-33.4.1116

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
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Galen P. Dively, Robyn Rose, Mark K. Sears, Richard L. Hellmich, et al.. "Effects on Monarch Butterfly Larvae (Lepidoptera: Danaidae) After Continuous Exposure to Cry1Ab-Expressing Corn During Anthesis" Environmental Entomology Vol. 33 Iss. 4 (2004) p. 1116 - 1125
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