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Article
Video-tracking and On-plant Tests Show Cry1Ab Resistance Influences Behavior and Survival of Neonate Ostrinia nubilalis Following Exposure to Bt Maize
Journal of Insect Behavior
  • Jarrad R Prasifka, United States Department of Agriculture
  • Richard L. Hellmich, Iowa State University
  • Andre LB Crespo, University of Nebraska-Lincoln
  • Blair D. Siegfried, University of Nebraska–Lincoln
  • David W. Onstad, University of Illinois at Urbana-Champaign
Document Type
Article
Publication Date
1-1-2010
DOI
10.1007/s10905-009-9190-3
Abstract

To examine how resistance to Bacillus thuringiensis (Bt) toxins influences movement and survival of European corn borer (Ostrinia nubilalis [Hübner]) neonates, the responses of Cry1Ab-resistant , -susceptible, and hybrid (F1) larvae were examined using two different techniques. First, using an automated video-tracking system, aspects of O. nubilalis movement were quantified in the presence of artificial diet incorporating 50% non-Bt or insect-resistant Cry1Ab maize tissue. Second, O. nubilalis dispersal and survival were measured 48–72 h after hatching on a Cry1Ab maize plant surrounded by two non-Bt maize plants. Video tracking indicated the presence of Cry1Ab tissue increased the total distance moved (m), time moving (%), and time away from the diet (%) for O. nubilalis while decreasing meander (degrees/cm). However, resistant larvae showed reduced movement and increased meander (≈localized searching) relative to susceptible or hybrid larvae on diet incorporating Cry1Ab tissue. Conversely, when placed onto Cry1Ab maize plants, resistant larvae were more likely than susceptible O. nubilalis to disperse onto adjacent non-Bt plants. The difference in on-plant dispersal seems to reflect greater survival after toxin exposure for resistant larvae rather than increased activity. These results suggest that simplified ‘Petri dish’ tests may not be predictive of larval movement among non-Bt and insect-resistant Bt maize plants. Because models of O. nubilalis resistance evolution incorporate various movement and survival parameters, improved data for on-plant behavior and survival of Bt- resistant , -susceptible, and hybrid larvae should help preserve the efficacy of transgenic insect-resistant maize.

Comments

This article is from Journal of Insect Behavior; 23 (2010); 1-11; doi: 10.1007/s10905-009-9190-3

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
Date Available
2014-08-28
File Format
application/pdf
Citation Information
Jarrad R Prasifka, Richard L. Hellmich, Andre LB Crespo, Blair D. Siegfried, et al.. "Video-tracking and On-plant Tests Show Cry1Ab Resistance Influences Behavior and Survival of Neonate Ostrinia nubilalis Following Exposure to Bt Maize" Journal of Insect Behavior Vol. 23 Iss. 1 (2010) p. 1 - 11
Available at: http://works.bepress.com/richard_hellmich/57/